Methylated & Non-methylated pUC19 DNA Set Chromatin Analysis The kit achieves high conversion

The kit achieves high conversion efficiency of unmodified cytosines into uracil

Purified DNA is RNA-free bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations

Simply add DNA/RNA Shield and Proteinase K to extract total RNA from any tissue

Yeast prepared with this kit can be transformed with both circular and linear DNAs

DNA/RNA is eluted into ≥50 µl of DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing

Methylated & Non-methylated pUC19 DNA Set Chromatin Analysis The kit achieves high conversionThe Methylated & Non methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers. The set is ideal as a spike in control to assess bisulfite conversion efficiency within the same reaction as the sample, or to produce known mixtures of methylated and non methylated DNA for assay calibration. The non methylated pUC19 DNA is pUC19 isolated from a methylation negative strain of bacteria (Dam, Dcm), and the methylated pUC19